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pbpF [2018-02-16 18:31:52]
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pbpF [2018-02-16 18:31:52]

penicillin-binding protein 2C
locus
BSU10110
pI
7.08
mw
79.10 kDa
protein length
714 aa Sequence Blast
gene length
2142 bp Sequence Blast
function
bifunctional glucosyltransferase/ transpeptidase
product
penicillin-binding protein 2C
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,083,851 → 1,085,995

    The protein

    Paralogous protein(s)

  • [protein|E50D4B30C2A0987A356C988AF8A4951C0CDF6C06|PbpD], [protein|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|PbpG], [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PonA]

    • Structure

  • [PDB|2OLU] (from Staphylococcus aureus, aa 40-610, 32% identity) [pubmed|17347437]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), prespore [Pubmed|15758244]
  • during vegetative growth: septal, low flourescence at periphery [pubmed|14731276]

    • Expression and Regulation

    Operons

    genes
    [gene|73275060537497E6A9859856C9F040763E36316B|pbpF]
    description
    [Pubmed|1459957]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8335642], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15758244], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed in mother cell and prespore [Pubmed|15758244]
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    Biological materials

    Mutant

  • BKE10110 (Δ[gene|73275060537497E6A9859856C9F040763E36316B|pbpF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCTCGCCTTT, downstream forward: _UP4_TAATGGAATTCGGCGATTTT
  • BKK10110 (Δ[gene|73275060537497E6A9859856C9F040763E36316B|pbpF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCTCGCCTTT, downstream forward: _UP4_TAATGGAATTCGGCGATTTT

    • GFP fusion

  • 2084 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|73275060537497E6A9859856C9F040763E36316B|pbpF]::pSG1491 (cat Pxyl-gfpa-[gene|73275060537497E6A9859856C9F040763E36316B|pbpF]1-335) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • References

  • 8335642,8606187,15758244,1459957,14731276,17347437